Phis2-p53
http://www.biofeng.com/zaiti/jiaomu/p53his.html WebbOur Matchmaker Gold Yeast One-Hybrid Library Screening System provides a simple and efficient method for identifying and characterizing novel protein-DNA interactions. All Matchmaker Gold Systems use Aureobasidin A resistance (AbA r) as a stringent, highly selective, and easy-to-use reporter.
Phis2-p53
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Webb实验步骤: 1.构建载体目的基因融合连入pGADT7-Rec2 (AD); 顺式作用元件连入pHis2或pHis2.1载体 (顺式元件可以只连接一个拷贝， 也可以连接3个串联的并测序确认无误)。. 2.验证两载体分别于对应的空载体共转时，有无激活转录报告基因情况，大多酵母苗株有毒 … Webb2.制备感受态并将pHis2-DNA转化进入感受态细胞； 3.SD/-Trp平板检测转化结果。 二、自激活检测 1.制备不同3-AT浓度梯度的SD/-His/-Trp平板，3-AT的浓度一般设定在50-100mM …
WebbBait pHIS2.1 containing repeats of your target sequence of interest Prey pGADT7-Rec2 vector containing a library gene Yeast Phenotypes His–, Leu–, or Trp– Requires histidine (His), leucine (Leu), or tryptophan (Trp) in the medium to grow; i.e., is auxotrophic for one (or more) of these specific nutrients Miscellaneous Webbp53-His2. Catalog No. PVT11251 Packing . p53-His2 Information. Function yeast Hybrid plasmid Resistance Kan Screen / Strain DH5alpha Culture temperature 37degrees …
Webb12 apr. 2024 · PHIS2-p53+pGAD53m SD-TL SD-TLH 阳性对照 3AT是酵母HIS3蛋白合成的竞争性抑制剂，用于抑制His3基因的泄漏表达。 实验结果如下： 图1：背景筛选结果 结果 … WebbpBIND pACT-MyoD pBIND-Id pG5 luc pCMV-BD pCMV-AD pBD-p53 pBD-NF−κB pAD-SV40T pAD-TRAF pFR-luc pGBKT7 pGADT7 pCL1 pGBKT7-53 pGADT7-T pGBKT7-Lam pACT2 AD Y187 AH109 pSos pMyr pSos MAFB pHis2 pHisSi-1 p53blue pGAG424 pLaczi p53his pGAD53m pBridge Y2H Gold Yeast strain Y187 pIND
Webb7 dec. 2024 · For Y1H assays, sequences including the MBS or ERE elements from the promoter of VvMYBPA1 or VvMYBPA2 were cloned into the pHis2 vector. Mutant MBS or ERE elements were used as a negative control. The ORF of VvMYB14 or VvERF104 was cloned into the pGADT7 vector.
Webbp53-His,pLacZ,pHis，pHisi-1. 1、长期保存的储存菌株以冷冻干燥保藏为主，特殊要求的菌种可用其它方法进行保存，保存期可长达几年至几十年。. 2、半储存菌株以含20%甘油的肉汤或液体石腊封存的半固体琼脂为主，在0℃以下保存，保存期为3－6个月。. 3、应用菌株 … crypt of hearts 2 hmhttp://www.labbase.net/Supply/SupplyItems-4229347.html crypt of hearts 2 guideWebb1 mars 2024 · The pHIS2-p53/pGADT7-Rec2-p53 and pHIS2-p53/pGADT7-Rec2-PtrHAT22 were used as the positive and negative control, respectively. 10 −1, 10 −2, and 10 −3 represent solution dilution ratio of transformed Y187 yeast cells. 4. Discussion. crypt of hearts dungeon locationWebb9 apr. 2024 · 目的：发现与A相互作用的motif 方法：以构建到pGADT7载体上的A基因为诱饵，筛选酵母单杂交motif文库，经过对阳性克隆的基因检测、DNA测序和比对分析，确定与pGADT7-A相互作用的motif。 材料： 1．诱饵克隆：pGADT7-A 2．诱饵载体：pGADT7 3．文库酵母质粒：motif 4．Clontech酵母单杂交系统 5．培养基 crypt of hearts helmetWebb1）准备质粒：构建pHis2-bait质粒→提取质粒； 2）制备Y187感受态细胞：YPDA平板活化→YPDA液体扩繁→制备酵母感受态细胞；或者直接购买Y187感受态细胞（CC306）； … crypt of heroes esoWebbFRFS-0353-2 For Epcos saw filter P/N B4959 REV 1.00 Electro-Photonics LLC reserves the right to modify drawings, configurations, specifications, and design at any time without … crypto-guider.com reviewWebb1 dec. 2024 · The pHIS2-p53/pGADT7-Rec2-p53 and pHIS2-p53/pGADT7-Rec2-PagWRKY75 co-transformed yeast cells were used as positive and negative control, respectively. 3.5. Generation of overexpression or knockdown plants for PagWRKY75. crypt of hearts gear