WebJun 1, 2015 · With an aim of enhancing the sensitivity of detection methods that use existing equipment and luminescent agents, we attempted to develop a new derivative by modifying a conventional biotinylated Phos-tag, BTL-104 [18], [19], [20].A comparison of several newly synthesized Phos-tag derivatives by means of Western blotting analyses of reference … WebFigure 1: (a) Structure of biotin-pendant Phos-tag ligand (BTL-104) and scheme for reversible capture of a phosphomonoester dianion (R-OPO 3 2–) by the Zn(II) complex of Phos-tag Biotin. (b) Schematic representation of ECL detection of phosphoproteins on a protein-blotting membrane by using Phos-tag Biotin.
The photostability of the commonly used biotin-4-fluorescein probe
WebTo investigate protein phosphorylation, phosphate-binding tag molecule “Phos-tag” was developed. In addition, various techniques and tools using Phos-tag such as Phos-tag SDS-PAGE, have been... WebApr 1, 2012 · In this study, our aim was to develop more-advanced applications for the detection of phosphopeptides and phosphoproteins by using several newly synthesized … r and l sounds
Highly sensitive detection of protein ... - Wiley Online Library
WebThe structure of the derivative BTL-104 is also shown for reference. The introduction of the long hydrophilic spacer [dodeca(ethylene glycol)] increases the fl exibility of the phosphate-binding... WebNov 26, 2024 · We have developed a technology known as Phos-tag that permits the analysis of phosphorylated biomolecules. Our Phos-tag technology utilizes a novel phosphate-binding tag molecule known as Phos-tag, which is capable of capturing phosphate monoester dianions ( R -OPO 32−) in aqueous solutions of neutral pH [ 4 ]. r and l sales corsicana